Fluorescence microscopes

There is nothing more important in this microscope category than excellent quality optics. Two classes of objectives are best suited for the daily, demanding analysis of stained cells. On the one hand there are fluorite objectives which are marketed under their brand names such as Neofluar or Neofluor, and the even more expensive class of plan apochromat objectives.

These objectives generally have very good to excellent colour correction and field flattening, so that the user gets a true-colour representation and sees no or only minor distortions at the image edges. Both facts contribute greatly to a microscope’s high resolution.

Objective magnifications from 2x to 60x or 100x are common.

Depending on the type of specimen, objectives that have been optimised during the design process for observation with or without a coverslip are used. In this case, there are also overlaps in the areas of application, especially at smaller magnifications.

Flexibly designed versions have a correction ring that allows adjustment to the coverslip thickness up to a setting for no coverslip.

A large object field, i.e. the size of the visible image, is very important for the observation of pathological and cytological specimens. Eyepieces with a large field number ranging from 23 to even 25 are ideally suited for these tasks. The field number is printed on the eyepiece after the eyepiece magnification - for example 10x/25.

Standardised fields or scales built into the eyepiece housing allow cells to be counted and measured.

In microscopy, LED illumination is now very common. It has a comparable colour rendering index to that of natural sunlight or artificial halogen light. It also ensures an almost continuously constant colour temperature at different light intensities - unlike under a halogen light, which appears whiter the brighter it shines.

A so-called light manager has become an extremely useful feature of modern microscopes. It stores the preferred light intensity for the respective objectives, so that the user does not need to manually adjust the light intensity each time the objective is changed.  In fact, objectives with higher magnification usually absorb light.

What makes a microscope pleasant to use? It is features such as low-seated rotary knobs for moving the stage, or a stage that is silky-smooth in its movement. However, many pathologists also prefer to manipulate their specimens directly with their fingers. In this case, a microscope model with a very low stage may be of interest. 

In the microscope head section, an ergonomically rotating tube is a great advantage in order to find the most comfortable position for microscopy. If you try it once you will never want to be without it again.